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Table 2 Primer sequences for plasmid construction

From: Dysregulation of the tumor suppressor Menin and its target Bach2 in HTLV-1 infection

Primer name

Direction

Sequences (5’ to 3’)

MEN1-FOR

Forward

ATTAT GAATTC tg ggc ggc ATG GGG CTG AAG GCC GCC CAG AAG AC

MEN1-REV

Reverse

ATTAT GCGGCCGC ta TCA GAG GCC TTT GCG CTG CCG CTT GAG

FRAG-FOR

Forward

ATTAT GGGCCC acc ATG GAC TAC AAA GAC GAT GAC

FRAG-REV

Reverse

ATTAT GAATTC c CTT GTC GTC ATC GTC TTT GTA GTC

BACH2-FOR

Forward

ATTAT GAATTC tg ggc ggc ATG TCT GTG GAT GAG AAG CCT GAC TC

BACH2-REV

Reverse

ATTAT GCGGCCGC tca CTA GGT ATA ATC TTT CCT GGG CTG

  1. GAATTC, GCGGCCGC, and GGGCCC sequences are the respective restriction sites for EcoRI, NotI, and ApaI
  2. Underlines indicate the FLAG-tag sequences