Table 2 Proteins identified in the present HIV-1 Gag affinity purification and found in at least one of the previously published HIV-1 Gag proteomic publications

MTDH

Metadherin

N/ER

Y

5

*

*

*

*

*

NAT10

N-acetyltransferase 10; RNA cytidine acetyltransferase

N

Y

4

*

*

*

*

ZC3HAV1

Zinc finger CCCH-type containing, antiviral 1

N/C

Y

4

*

*

*

*

ABCF1

ATP binding cassette subfamily F member 1

C/N

Y

3

*

*

*

CCT8

Chaperonin containing TCP1 subunit 8; T-complex protein 1 subunit theta

Ck/C

Y

3

*

*

*

CDC5L

Cell division cycle 5 like

C/N

Y

3

*

*

*

HNRNPM

Heterogeneous nuclear ribonucleoprotein M

N

Y

3

*

*

*

MAP4

Microtubule associated protein 4

Ck

Y

3

*

*

MOV10

RISC complex RNA helicase

N/C

Y

3

*

*

*

NUFIP2

FMR1 interacting protein 2; Nuclear fragile X mental retardation-interacting protein 2

N/C

N

3

*

*

*

PLRG1

Pleiotropic regulator 1

N

N

3

*

*

*

PRPF3

Pre-mRNA processing factor 3; U4/U6 small nuclear ribonucleoprotein Prp3

N

N

3

*

*

*

PRPF4

Pre-mRNA processing factor 4; U4/U6 small nuclear ribonucleoprotein Prp4

N

N

3

*

*

*

RBM14

RNA binding motif protein 14

N/C

Y

3

*

*

*

RPL37A

60S ribosomal protein L37a

C/S/N

Y

3

*

*

*

SRPK1

SRSF protein kinase 1

N/C/ER

Y

3

*

*

*

SRSF3

Serine/arginine rich splicing factor 3

N/C

Y

3

*

*

*

SRSF7

Serine/arginine rich splicing factor 7

N/C

Y

3

*

*

*

TMPO

Thymopoietin; Lamina-associated polypeptide 2, isoform alpha

N

Y

3

*

*

*

TOP1

DNA topoisomerase I

N

Y

3

*

*

*

ALYREF

Aly/REF export factor; THO complex subunit 4

N/C

Y

2

*

*

ATAD3B

ATPase family, AAA domain containing 3B

M

Y

2

*

*

C7orf50

Chromosome 7 open reading frame 50

N

N

2

*

*

CHTOP

Chromatin target of PRMT1

N

N

2

*

*

DAP3

Death associated protein 3; 28S ribosomal protein S29, mitochondrial

M

Y

2

*

*

DDX24

DEAD-box helicase 24

N/Me

Y

2

*

*

DDX27

DEAD-box helicase 27

N

Y

2

*

*

DDX47

DEAD-box helicase 47

N

Y

2

*

*

DHX30

DExH-box helicase 30

M/C

Y

2

*

*

DIMT1

DIM1 dimethyladenosine transferase 1 homolog

N

Y

2

*

*

DKC1

Dyskerin pseudouridine synthase 1; H/ACA ribonucleoprotein complex subunit

N/C

Y

2

*

*

EIF2S1

Eukaryotic translation initiation factor 2 subunit alpha

C

Y

2

*

*

ELAVL1

ELAV like RNA binding protein 1

N/C

Y

2

*

*

EXOSC2

Exosome component 2

N/C

Y

2

*

*

FAM120A

Family with sequence similarity 120A; Constitutive coactivator of PPAR-gamma-like protein 1

PM/C

N

2

*

*

FBL

Fibrillarin; rRNA 2'-O-methyltransferase

N

N

2

*

*

FXR1

FMR1 autosomal homolog 1; Fragile X mental retardation syndrome-related protein 1

C

Y

2

*

*

G3BP2

G3BP stress granule assembly factor 2; Ras GTPase-activating protein-binding protein 2

C

Y

2

*

*

GRSF1

G-rich RNA sequence binding factor 1

M

Y

2

*

*

GRWD1

Glutamate rich WD repeat containing 1

N

N

2

*

*

GTF3C2

General transcription factor IIIC subunit 2

N

Y

2

*

*

H1FX

H1 histone family member X

N

Y

2

*

*

HIST1H1E

Histone cluster 1 H1 family member e

N

Y

2

*

*

HNRNPDL

Heterogeneous nuclear ribonucleoprotein D like

N/C

Y

2

*

*

HNRNPH3

Heterogeneous nuclear ribonucleoprotein H3

N

Y

2

*

*

HNRNPR

Heterogeneous nuclear ribonucleoprotein R

ER/N/C

Y

2

*

*

HNRNPUL1

Heterogeneous nuclear ribonucleoprotein U like 1

N

Y

2

*

*

IARS

Isoleucyl-tRNA synthetase

C

Y

2

*

*

IGF2BP1

Insulin like growth factor 2 mRNA binding protein 1

N/C

Y

2

*

*

IGF2BP3

Insulin like growth factor 2 mRNA binding protein 3

N/C

Y

2

*

*

ILF2

Interleukin enhancer binding factor 2

N/C

Y

2

*

*

ILF3

Interleukin enhancer binding factor 3

N/C

Y

2

*

*

LSM12

LSM12 homolog

C/N

Y

2

*

*

MKI67

Marker of proliferation Ki-67

N

Y

2

*

*

MRPS9

Mitochondrial ribosomal protein S9

M

Y

2

*

*

MRPS23

Mitochondrial ribosomal protein S23

M

Y

2

*

*

MRPS27

Mitochondrial ribosomal protein S27

M/C

N

2

*

*

MRPS31

Mitochondrial ribosomal protein S31

M

N

2

*

*

MRPS34

Mitochondrial ribosomal protein S34

M

N

2

*

*

NOP2

NOP2 nucleolar protein; Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase

N

N

2

*

*

NOP16

NOP16 nucleolar protein

N

N

2

*

*

PES1

Pescadillo ribosomal biogenesis factor 1

N

N

2

*

*

PDCD11

Programmed cell death 11

N

Y

2

*

*

PRRC2C

Proline rich coiled-coil 2C

C

Y

2

*

*

PTCD3

Pentatricopeptide repeat domain 3

M

Y

2

*

*

PURA

Purine rich element binding protein A

N

Y

2

*

*

QARS

Glutaminyl-tRNA synthetase

C

Y

2

*

*

RBM10

RNA binding motif protein 10

N

Y

2

*

*

RFC1

Replication factor C subunit 1

N

Y

2

*

*

RFC5

Replication factor C subunit 5

N

Y

2

*

*

RPL5

60S ribosomal protein L5

C/N

Y

2

*

*

RPL15

60S ribosomal protein L15

Me

N

2

*

*

RPS24

40S ribosomal protein S24

C/ER/N/Me

Y

2

*

*

RPS28

40S ribosomal protein S28

C/ER

Y

2

*

*

RSL1D1

Ribosomal L1 domain containing 1

N

Y

2

*

*

SERBP1

SERPINE1 mRNA binding protein 1

N/C

N

2

*

*

SNRPA

U1 small nuclear ribonucleoprotein polypeptide A

N

Y

2

*

*

SPTAN1

Spectrin alpha, non-erythrocytic 1

Ck

Y

2

*

*

STRBP

Spermatid perinuclear RNA binding protein

C

Y

2

*

*

TRA2B

Transformer 2 beta homolog

N

Y

2

*

*

UPF1

RNA helicase and ATPase

N/C

Y

2

*

*

WDR3

WD repeat domain 3

N

Y

2

*

*

XRN1

5'–3' exoribonuclease 1

C

Y

2

*

*

ABLIM1

Actin binding LIM protein 1

Ck/C

N

1

*

ACTN4

Actinin alpha 4

Ck/C/N

Y

1

*

AFG3L2

AFG3 like matrix AAA peptidase subunit 2

M

Y

1

*

AHNAK

Neuroblast differentiation-associated protein

N

Y

1

*

ANKRD17

Ankyrin repeat domain 17

N/C

Y

1

*

ASUN

(INTS13)

Asunder, spermatogenesis regulator;

Integrator complex subunit 13

N/C

N

1

*

BAG2

BCL2 associated athanogene 2

Ck/C

N

1

*

BAZ1A

Bromodomain adjacent to zinc finger domain 1A

N

Y

1

*

BAZ1B

Bromodomain adjacent to zinc finger domain 1B

N

Y

1

*

BSG

Basigin

PM/ER

Y

1

*

BUB3

Mitotic checkpoint protein

N

N

1

*

BYSL

Bystin like

N/C

N

1

*

C1QBP

Complement C1q binding protein

N/S/PM/M/C

Y

1

*

CCAR2

Cell cycle and apoptosis regulator 2

N/C

Y

1

*

CCDC86

Coiled-coil domain containing 86

N

N

1

*

CCT4

Chaperonin containing TCP1 subunit 4

Ck/C

Y

1

*

CCT5

Chaperonin containing TCP1 subunit 5

Ck/C

Y

1

*

CCT6A

Chaperonin containing TCP1 subunit 6A

C

Y

1

*

CCT7

Chaperonin containing TCP1 subunit 7

C

Y

1

*

CD3EAP

CD3e molecule associated protein; DNA-directed RNA polymerase I subunit RPA34

N

N

1

*

CDK12

Cyclin dependent kinase 12

N

N

1

*

CLTC

Clathrin heavy chain

Ck/C

Y

1

*

CORO1C

Coronin 1C

PM/Ck/Me

N

1

*

CTNNB1

Catenin beta 1

Ck/PM/N/C

Y

1

*

DDX20

DEAD-box helicase 20

N/C

Y

1

*

DDX31

DEAD-box helicase 31

N

N

1

*

DDX46

DEAD-box helicase 46

N/Me

Y

1

*

DNAJA3

DnaJ heat shock protein family (Hsp40) member A3

C/M/PM

N

1

*

DNMT1

DNA methyltransferase 1

N

Y

1

*

ECPAS (KIAA0368)

Ecm29 Proteasome Adapter and Scaffold

ER/N/Ck

N

1

*

EEF1B2

Eukaryotic translation elongation factor 1 beta 2

C

N

1

*

EXOSC4

Exosome component 4

C/N

N

1

*

FAU

Ubiquitin like and ribosomal protein S30 fusion

S/N/C

Y

1

*

FIP1L1

Factor interacting with PAPOLA and CPSF1

N

Y

1

*

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

C/Ck/N/Me

Y

1

*

GLYR1

Glyoxylate reductase 1 homolog

N

N

1

*

GPATCH4

G-patch domain containing 4

N/C

Y

1

*

HIST1H1A

Histone cluster 1 H1 family member a

N

Y

1

*

HIST2H2BF

Histone cluster 2 H2B family member f

N

Y

1

*

HNRNPUL2

Heterogeneous nuclear ribonucleoprotein U like 2

N

N

1

*

HP1BP3

Heterochromatin protein 1 binding protein 3

N

N

1

*

INIP

INTS3 and NABP interacting protein; SOSS complex subunit C

N

N

1

*

INTS9

Integrator complex subunit 9

N

N

1

*

ITPR1

Inositol 1,4,5-trisphosphate receptor type 1

ER/S

Y

1

*

KIF4A

Chromosome-associated kinesin family member 4A

Ck/C/N

Y

1

*

KIF23

Kinesin family member 23

N

Y

1

*

KLC2

Kinesin light chain 2

Ck/Me

N

1

*

KRT2

Keratin 2

Ck/C/S/N/PM

Y

1

*

KRT16

Keratin 16

Ck/C/E/N

Y

1

*

KRT19

Keratin 19

Ck/C/E/PM

Y

1

*

LARP4

La ribonucleoprotein domain family member 4

C

Y

1

*

LARP7

La ribonucleoprotein domain family member 7

N

Y

1

*

MAPK1

Mitogen-activated protein kinase 1

Ck/C/N

Y

1

*

MBD2

Methyl-CpG binding domain protein 2

N

Y

1

*

MED26

Mediator complex subunit 26

N

Y

1

*

MEPCE

7SK snRNA methylphosphate capping enzyme

N

Y

1

*

MINA (RIOX2)

MYC induced nuclear antigen; Ribosomal oxygenase 2

N

N

1

*

MRPL10

39S mitochondrial ribosomal protein L10

M

Y

1

*

MRPL53

Mitochondrial 39S ribosomal protein L53

M

Y

1

*

MRPS14

Mitochondrial 28S ribosomal protein S14

M

N

1

*

MRPS22

Mitochondrial 28S ribosomal protein S22

M

N

1

*

NOC4L

Nucleolar complex associated 4 homolog

N

N

1

*

PABPN1

Poly(A) binding protein nuclear 1

N/C

Y

1

*

PAXBP1

PAX3 and PAX7 binding protein 1

N

Y

1

*

PBRM1

Polybromo 1

N

Y

1

*

PCBP1

Poly(rC) binding protein 1

C/N

Y

1

*

PPIH

Peptidylprolyl isomerase H

N/C

Y

1

*

PRRC2B

Proline rich coiled-coil 2B

N/C

N

1

*

PSMC3

Proteasome 26S subunit, ATPase 3

N/C

Y

1

*

PTBP1

Polypyrimidine tract binding protein 1

N

Y

1

*

PTRF (CAVIN1)

Polymerase I and transcript release factor; Caveolae-associated protein 1

C/N/ER/M/PM

N

1

*

PUM1

Pumilio RNA binding family member 1

C

N

1

*

RBM7

RNA binding motif protein 7

N

Y

1

*

RBM26

RNA binding motif protein 26

N

Y

1

*

RCC1

Regulator of chromosome condensation 1

N/C

Y

1

*

RNF213

E3 ubiquitin-protein ligase; ring finger protein 213

C

Y

1

*

RRP9

U3 small nucleolar RNA-interacting protein 2

N

Y

1

*

RSBN1L

Round spermatid basic protein 1 like; Lysine-specific demethylase

N

Y

1

*

SDF2L1

Stromal cell derived factor 2 like 1

ER

Y

1

*

SEC24B

SEC24 homolog B, COPII coat complex component

C/ER

N

1

*

SLC25A3

Solute carrier family 25, member 3

M

Y

1

*

SLC25A4

Solute carrier family 25 member 4; ADP/ATP translocase 1

M/Me

Y

1

*

SLC25A11

Solute carrier family 25 member 11; Mitochondrial 2-oxoglutarate/malate carrier protein

M

Y

1

*

SMARCA4

SWI/SNF related, matrix associated, actin dependent regulator of chromatin; Transcription activator BRG1

N

Y

1

*

SMARCC2

SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 2

N

Y

1

*

SMC4

Structural maintenance of chromosomes 4

C/N

Y

1

*

SMN2

Survival of motor neuron 2, centromeric

N/C

N

1

*

SNRPD2

Small nuclear ribonucleoprotein D2 polypeptide

C/N

Y

1

*

SPIN1

Spindlin 1

N

Y

1

*

SPOUT1

SPOUT domain containing methyltransferase 1

Ck

Y

1

*

SRP9

Signal recognition particle 9

C

Y

1

*

SUPT16H

SPT16 homolog, facilitates chromatin remodeling subunit

N

Y

1

*

TAB1

TGF-beta activated kinase 1 (MAP3K7) binding protein 1

C/Me/N

N

1

*

TCEB1 (ELOC)

Transcription elongation factor B subunit 1; Elongin-C

N

Y

1

*

TCP1

t-complex 1

C/Ck

Y

1

*

TPM1

Tropomyosin 1 (alpha)

Ck

Y

1

*

TPM3

Tropomyosin 3

Ck

Y

1

*

TRIP12

Thyroid hormone receptor interactor 12; E3 ubiquitin-protein ligase

N

N

1

*

TRRAP

Transformation/transcription domain associated protein

N

Y

1

*

TUBB6

Tubulin beta 6 class V

Ck

Y

1

*

U2AF1

U2 small nuclear RNA auxiliary factor 1; Splicing factor U2AF 35 kDa

N

Y

1

*

UBAP2L

Ubiquitin associated protein 2 like

C

Y

1

*

URB1 (NPA1)

Nucleolar pre-ribosomal-associated protein 1

N

Y

1

*

UTP20

UTP20, small subunit processome component

N

N

1

*

WDR5

WD repeat domain 5

N

Y

1

*

WDR82

WD repeat domain 82

N

Y

1

*

ZC3H4

Zinc finger CCCH-type containing 4

C/N

N

1

*